Quantitative determination of urea concentrations in cell culture medium
نویسندگان
چکیده
منابع مشابه
Quantitative determination of urea concentrations in cell culture medium.
Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied...
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ژورنال
عنوان ژورنال: Biochemistry and Cell Biology
سال: 2009
ISSN: 0829-8211,1208-6002
DOI: 10.1139/o09-011